PRKN Antibodies

AND PRKN ELISA kits, PRKN Proteins

Many biological assays use antibodies to detect PRKN, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with PRKN in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop PRKN antibodies...

We validate the specificity of these antibodies to PRKN by testing them on tissues known to express PRKN positively and negatively. Browse below to find the PRKN antibody that suites your experiment. We have 7 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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PRKN Infographic by Boster Bio

All PRKN Products

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Specificity

Applications

Price

Anti-Parkin/PRKN Antibody Picoband®

4 Q&As

Human, Mouse, Rat

IHC, ICC, WB

$370
Cat # PB9307
100 μg/vial

Parkin Colorimetric Cell-Based ELISA Kit

Human

ELISA

$480
Cat # EKC1440
1 kit, containing one 96-well plate and all necessary reagents

Anti-PARK2 (T125) PRKN Antibody

Human, Mouse, Rat

IHC

$405
Cat # A31800T125
100ul

Anti-Parkin (phospho-S131) PRKN Antibody

Human

$482
Cat # A31800S131
100ul

Anti-Phospho-Parkin (S131) PRKN Antibody

Human

ELISA, IHC

$380
Cat # A31800S131-1
100ul

Human Parkin Recombinant Protein

SDS-PAGE, Denatured

$250
Cat # PROTO60260
Starting from 20ug

Parkin (PARK2) (NM_013987) Human Over-expression Lysate

Human

$960
Cat # LS005071
100 µg

PRKN Overview

Facts about E3 ubiquitin-protein ligase parkin.

PRKN 3D structure. Source: alphafold

E3 ubiquitin-protein ligase parkin (PRKN)

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPT5, TOMM20, USP30, ZNF746 and AIMP2 (PubMed:10973942, PubMed:10888878, PubMed:11431533, PubMed:12150907, PubMed:12628165, PubMed:16135753, PubMed:21376232, PubMed:23754282, PubMed:23620051, PubMed:24660806, PubMed:24751536).
Mediates monoubiquitination and'Lys-6','Lys-11','Lys-48'-connected and'Lys-63'-linked polyubiquitination of substrates depending on the circumstance (PubMed:19229105, PubMed:20889974, PubMed:25621951).

Participates in the elimination and/or detox of abnormally folded or damaged protein by mediating'Lys-63'-linked polyubiquitination of misfolded proteins like PARK7:'Lys-63'- linked polyubiquitinated misfolded proteins are then recognized by HDAC6, resulting in their recruitment to aggresomes, followed by degradation (PubMed:17846173, PubMed:19229105). Mediates'Lys-63'- linked polyubiquitination of a 22 kDa O-linked glycosylated isoform of SNCAIP, possibly playing a role in Lewy-body formation (PubMed:11590439, PubMed:11431533, PubMed:19229105, PubMed:11590439, PubMed:15728840).

Gene Information

Human
Gene Name:	PRKN
Uniprot:	O60260
Entrez:	5071

Family

Belongs to:

RBR family

Alternative Names

AR-JP; E3 ubiquitin ligase; E3 ubiquitin-protein ligase parkin; EC 6.3.2.-; LPRS2; PARK2; parkin 2; Parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2, E3 ubiquitin protein ligase (parkin); PDJ; PDJjuvenile) 2, parkin; PRKN

Molecular Weight

Mass (kDA):
51.641 kDA

Genomic Context


Human

Location:	6q26
Sequence:	6; NC_000006.12 (161347417..162727802, complement)

Tissue Specificity

Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis. Found in serum (at protein level).

Subcellular Localizations

Cytoplasm, cytosol. Nucleus. Endoplasmic reticulum. Mitochondrion. Mainly localizes in the cytosol. Co-localizes with SYT11 in neutrites. Co-localizes with SNCAIP in brainstem Lewy bodies. Mitochondrial localization gradually increases with cellular growth. Also relocates to dysfunctional mitochondria that have lost the mitochondrial membrane potential; recruitment to mitochondria is PINK1-dependent.